H�\��n�@E�|E/�E� EU"!K~$���x�0���Am���O_n����`�|Eu������\�3��O�ԇ.��p��wG�C�,\�����^�1�S��~��eNCV�.��n^�xw�n8��,�;�pv��G�n���/>Ln�V+��S��o����x��eO�.���S�����}������i��_Ǧ�� g�Ջt�\���U�C����d��Ծ71�,^,�)�o������_��-Q[��DmY�pI.�BpE��JV�� �B~�����\A�0W�+�� 0000061592 00000 n h��Xmo�6�+����$�S@��E�u�v�Au�D�-��6�~w'��9 �� �E�{ӑ<>G�Ʋ���8&�Y0�O��A�3i2�O�9ɸ�9ń+�L3��&5'�2i�B�1���8�3-g�������T� ��y${�2������ŦʎN���zY.�����ݹ;?e�6UvqWe�_۟fm�Vټ$��]�wx��7�i�s�M˻WU}s�2cD�cթ$�N�͆I������h���@���&1�)�iO�e����^VvV}a�W˲��tg��B (H~>;� լ]W��6�E�dT�g��rQ�'�͢by6k��/��� ��]�w�j�}�cP��qN�d���|u]77ه��4�:�>�כvz[��e��]���doKo"��f�?���� %3�������BA��$tΤ�0��iX0$�Q�]�C"�Q���n}�"�%{�H)� trailer <<2A943EA66B8C11DB91B0000A95D99110>]>> startxref 0 %%EOF 235 0 obj<>stream 0000227407 00000 n $�� �� $X�AD�8sb� uj �'H" 0000023299 00000 n �V(-KD� {&�瑂�"���+ ~�QNyC4^�ޘ�`�|��cNs��w�1����S�| 4- Know how to prepare all types of buffer solutions . x�b```b``�e`e``ndd@ A6v�@N���"b1;���e��f=�f���^�ƃÇզ�vpe#���. 92 0 obj <> endobj 125 0 obj <>/Encrypt 93 0 R/Filter/FlateDecode/ID[<8DEF34DA1DB348AA912F1AD605BCEACF><99E998569A3047119B179B77E13CC274>]/Index[92 61]/Info 91 0 R/Length 139/Prev 1267682/Root 94 0 R/Size 153/Type/XRef/W[1 3 1]>>stream Phosphate buffered saline contains sodium chloride (NaCl) and dibasic sodium phosphate (Na2PO4). A buffer is an aqueous solution consisting of a mixture of a weak acid and its salt ( acidic buffer ) or a weak base and its salt ( basic buffer ) . �EQ����E�/��4��,C��v A�y�.��Ÿ�� ����=�T��f��F:|�fڰ 0000056617 00000 n 0�)H���� ����=!`� 0000016093 00000 n 0000009388 00000 n ʳ�B�� 0000002241 00000 n 0000003444 00000 n H���[O\����W�#Vԇ�_�O���dFFʃE�i# ����] �L2�,sz�SU���ڻ��:�l��ܞ~��ܸ÷���>v����}t5�%��rKn�(�nWs�,;|�λw�\��B*5:ϿZ�r�9f��K�iԖܺ-%pnq�����;�^߬~X}��?�����s��������ޕ��+q�9�B��X��R[�#��LaI�����ڃ���Ԏ��&���G'��?�d��I���Ɠ�U�����V�o�����nu�Ml�N~\�i�������Rn����ׇ��9�����O�[��Pv'���:N;[���|{{'ſI��`�s3v��0���\lo���:��˻��o>�ߞ��n��K.�������������f(v�"�k|oC�Fr����������b��;������G��̷�����������Ͽ����|��2�垩�g mi%�V����z�+�|��wy�U���8Rik����%�����ӟ�O]!���,Yr|�����˫���n{��r��é��!I��f���yJy�*��g��������C&g�5��:w�?�����f��oO�|Cñ�V�FCMK,�嶤\�Q՞^�ٻ_�7�-�p��VSm�`_8�#N�8|I�G��=�aUK-Pg����Жy�a�R�X��Le�Z���&��YS���4��K��2K#��K�q�>�D ���ZC�-�0 �!�����u^r��� formulation and storage, buffers ensure optimal biological activity and stability of a drug product at the targeted storage temperature over the duration required for clinical or commercial use. 0000006349 00000 n 0000001523 00000 n %���� BIOLOGICAL BUFFERS Many chemical reactions are affected by the acidity of the solution in which they occur. Effective Available pack size Assay Code pH range (by Titration) 66822 ACES Buffer extrapure 7365-82 -4 6.1 - 7. Preparation and Use of Buffers in Biological Systems. Bicine buffer. We are frequently asked questions on the use of buffers that we offer to research laboratories. endstream endobj startxref 0000001640 00000 n 1944 0 obj <> endobj <>>> 0000002378 00000 n Many buffers are available today. 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Ƙ�h=����բfuTo�����ee��*�$iֈ�B*͡��Gk�S"�PU+�bz����j�TAa�l��5 Biological Buffers . ���Τ�nw>Ljv���@�ߨ7 +��NOú��c����(��YlY�rb��~ȟ�`U��)�zH���5�̗���)VBcK��H�K�$�>��q�G�!���Y�a �fU�o�9Ps@��GW��9��Z�`�#�:e���qoW1L�$#�?�. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent changes in the pH of a solution. Introduction . �!��z8��a&P�����F�y� h�bbd``b`� 0000008258 00000 n W�UK��t4Ey���s����| P�(��t���������O��N���. 0000033833 00000 n 0 Useful pH range: 7.6 - 9.0. pKa (25°C): 8.26. �!�p���P��N��&B�=x��m�ठ������9� �h�A+�N��(�I����CZ ư����zع�C����t.��^n�~�! 0000048949 00000 n This publication is a part of our continuing commitment to provide useful product information and exceptional service … �j �)���@�k2�h:A �@�7����a>�xF҈��� �/8 0000036810 00000 n Molecular weight: 163.2 g/mol. x�b```b``�������� ̀ �@16� @8,�e��Z"����A��q� �������m��h��� ^ Tx����{�^�����}o{�z���Ɋn~�s�RJL��ͩ}!��6��f��]�E$"�c�I�8����yt~�x�4� �̢7Jh����z�^#c+\����2o&�b0��%�D$a�ԥ��I�OD�M]7�L�iV�Ta���VI07�Rm^�T����NRs���-�4�!�5Mbn�!c^�8K1�! endobj It may also contain 0000029470 00000 n 0000227016 00000 n 0000003047 00000 n �q�+�G{_?������ F�?p��A#�[�0��G> �`z�Ӕ��Uȋ���%K2��nb��2����k�UZ��H����-�d��������cz��O�ov�� �K3B��1y(��,v�*��W�5 �Zn�: �H �M�����u].������{\5/��'��� U�k쟦PR�ƀB-�"�_�i�"$�z�>OQ���@�s�{x���7�/܀�.l$�����~�/���t#�z���z� 0000006949 00000 n Biological Buffers . )�B���\����"-1�6۩�n�����!��U��p�+�Y��`����]��1��+�M�WF5�۱�_ �&. 0000003830 00000 n 0000003719 00000 n ���弬F�$5�S3tO��%��h�l�(��B�3t����9�ƗU��L3!�G26ˆ�df�~��2����w�M�~�99WÜ�P�S�;!�8�'��ᮝZ�^����0b��c�5�o����ʼn�f � Gƛu8�d0Of5����IG�:�����Q��N;��Ɔ��ì�"� ���n�}g��/���2O������d���b67@əI�bf�'��҇�)�dԘ��5�Z�ë�ESV�v��Ծ�+
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