The data suggest that tetrathionate-forming heterotrophic bacteria, in particular those belonging to the P. stutzeri ‘superspecies’, can play a much more significant role in the biogeochemical cycles than was previously recognized. J. x�c```b``�f`e`�gd@ A�� E�G�$X�V�>Xe���7G���j��'�(I. The Ribosomal Database Project [25] and GenBank [26] were searched for sequences of closely related strains. Group 1 strains (an example is given in Table 4) did not accumulate nitrite during the anaerobic reduction of nitrate, but produced substantial amounts of N2O when incubated anaerobically with nitrite and thiosulfate. Tuovinen J Biol Chem 193:265–275, PubMed The absorption maxima typical of this nitrite reductase (γ 460–470 nm; α 610–615 and 660–670 nm) were not observed in spectra made on similar protein samples from the group 2 isolates. Teske 0000063123 00000 n Among the organic compounds utilized as carbon and energy sources were: organic acids including acetate, succinate, malate, α-ketoglutarate, citrate, fumarate, lactate, pyruvate, propionate, butyrate, gluconate, glyoxylate; alcohols including ethanol, propanol, glycerol; hexoses –d-glucose, d-fructose and d-maltose; amino acids and amines including i-alanine, l-glutamate, l-glutamine, l-aspartate, l-asparagine, l-proline and l-leucine. The kinetics of the reduction of tetrathionate imply a simultaneous interaction of tetrathionate and thiosulphate on the reductase molecule. If the nitrate concentration was limiting, acetate was preferentially oxidized. While this might indicate the presence of a copper-containing nitrite reductase, rather than cytochrome cd1, this seems unlikely in view of the failure of DDC to inhibit nitrite reduction. Google Scholar, Kelly DP, Chambers LA, Trudinger PA (1969) Cyanolysis and spectrophotometric estimation of trithionate in mixture with thiosulfate and tetrathionate. During the first phase, a rapid growth of heterotrophic, red-colored, polymorphic rods occurred with the concomitant oxidation of thiosulfate to tetrathionate. 0000001458 00000 n %PDF-1.2 R.A. /Prev 266297 I went to a Thanksgiving dinner with over 100 guests. Growth and thiosulfate oxidation in anaerobic batch culture of strain TG 3 with acetate(10 mM) and nitrate (20 mM). These experiments also demonstrated that the culture could not gain sufficient energy from thiosulfate oxidation to increase the growth yield observed with acetate alone. The oxidation of sulphide in cell-free extracts proceeds most likely to polysulphanes or to elemental sulphur, depending on the conditions. These molecular differences exclude strain TG 3 from the species [29]. Why does diethyl ether have the smallest dipole? Some strains converted thiosulfate to tetrathionate equally well with nitrite, nitrate and N2O as electron acceptors. An evolving electronic resource for the microbiological community. Jannasch It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. /Size 90 Was it a consequence of COVID-19? P. 0000019355 00000 n 0000018428 00000 n The observed preference for N2O as an electron acceptor for anaerobic thiosulfate oxidation to tetrathionate during organotrophic growth could be explained by the high redox potential of N2O/N2, compared with the nitrate/nitrite and nitrite/N2O couples (ΔE0′=1.36, 0.43 and 0.36 V, respectively), and assuming a relatively high value for the S2O32−/S4O62− pair (about 0.1 V). Sugars were not fermented. ‘Flavobacterium lutescens’ was able to grow anaerobically with acetate and nitrate, nitrite or N2O as electron acceptors. Cytochrome cd1 (nitrite reductase) was not expressed under aerobic conditions in any of the isolates from either group. The isolates also matched all P. stutzeri genomovar signature nucleotides [32], with the exception of an adenosine replaced by a guanosine in E. coli position 1036 of strain ChG 5-3 and in E. coli position 278 of strain BG 2. Li https://doi.org/10.1016/S0168-6496(99)00045-8. Ghosh W(1), Roy P. Author information: (1)Department of Microbiology, Bose Institute, Kolkata, India. The experiments with washed cells aimed to eliminate any potential influence of organic electron donors, and to clarify the roles of the different stable nitrogen oxides in anaerobic thiosulfate oxidation.
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