pediococcus acidilactici infection

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The quantitative primers for ETEC K88 enterotoxin gene. 0000071607 00000 n 0000004691 00000 n 326 0 obj <> endobj P25 could colonize in the intestinal canal of nematodes. 0000075551 00000 n Some genes’ abundances related to the innate immune response in C. elegans were significantly reduced due to K88 infection, but this was reversed by P25. 0000172750 00000 n Subscription will auto renew annually. Abstract. The oligonucleotide primers of eight pairs of candidate genes (kin-1, hsp-70, sek-6, gpa-12, Y105C5A.24, atf-5, ver-3, and ras-2) and reference genes (β-actin) are shown in Table 2. Copyright © 2020 Keqin Tan et al. 0000076507 00000 n M. All authors approved the final version of the article, including the authorship list. 0000170790 00000 n In: Sneath PHA, Mair SN, Sharpe ME, Holt JG (ed): Bergey's manual of systematic bacteriology. 0000169060 00000 n 0000167348 00000 n We sequenced the intergenic spacer (IGS) region target within the rRNA gene complex, which also was able to give reliable results using comparison analysis in the GenBank database. Archives of Oral Biology 1986, 11: 967–972. trailer 0000172276 00000 n In this study, results demonstrated that nematodes treated with P25 had a significant resistance to infection by K88 through multiple mechanisms. Legend represents the log2 (fold change) of gene abundance; the redder the color, the higher the gene expression, and conversely, the bluer the color. In the previous section, we found that P25 significantly prolonged the lifespan of K88-infected nematodes. The Etest method (bioMérieux, Durham, NC) described by Huang et al. ; validation, X-Y. After culturing at 37°C for 20 h, the bacteria on the surface of the nematodes were counted (). The above results suggested that the inhibitory effect of P25 on estB and elt may primarily occur during the initial stages of infection in vivo (Figure 5). Please enable it to take advantage of the complete set of features! The reaction mixture was then incubated at 42°C for 2 min; then, 1 μL PrimeScript RT Enzyme Mix I, 1 μL RT Primer Mix, 4 μL 5×PrimeScript Buffer 2, and 4 μL RNase-free dH2O were added to the reaction tubes for the next incubation at 37°C for 15 min, then 85°C for 5 sec. 0000080187 00000 n The adapters, low-quality reads (), or too many (undetermined base ) reads in the original sequencing data were filtered out to obtain clean reads. Pediococci exert antagonism against pathogens through the production of lactic acid and secretion of bacteriocins, bacteria-killing compounds, known as pediocins. The results indicated that the differential expression patterns of genes relating to the MAPK pathway as measured by RNA-seq analysis followed the same trend in abundances detected by qRT-PCR (Figure 11), which showed that the RNA-seq results were reliable. 0000073624 00000 n 0000167287 00000 n 0000167424 00000 n A 32-year-old male with a history of short gut syndrome following a small bowel transplant 1 year prior with severe rejection requiring explantation 3 weeks after transplant presented with fever (102°F), rigors, and dyspnea of 3 days' duration. Therefore, our results could also further supplement that the inhibition of pathogens by LAB may be species diverse in host cell colonization. and K-Q. Previous studies have shown that lactic acid bacteria (LAB) have great potential in promoting health and resisting pathogenic infections; however, relatively little research has been done on the Pediococcus genus of LAB. 0000075945 00000 n 2010 Nov;1(4):439-45. doi: 10.3920/BM2010.0025. Another main virulence factor of ETEC is enterotoxins [66]. P. acidilactici is also known to prevent colonization of the small intestine by pathogens such as Shigella spp., Salmonella spp., Clostridium difficile, and Escherichia coli among small animals. On the 2nd day of infection, the abundance of estB and elt in PR was 2.17 and 0.79 times lower than that in AT ( and ), respectively. The worms treated with OP50 served as negative controls (CK) while attacked group (AT) worms were treated with OP50 for 20 h; then, as much bacteria as possible were removed from the body surface and gut by submerging in 1 mL M9 medium (0.3% KH2PO4, 0.6% Na2HPO4, 0.5% NaCl, and 0.1% 1 M MgSO4) containing 20 μg/mL levamisole suspension for one minute, six times, and transferred to the K88 treatment. qRT-PCR assays were performed using the C1000 Touch thermal cycler and iTaq Universal SYBR Green Supermix (Bio-Rad, Shanghai, China). 0000168314 00000 n Two reviews are available in the literature that report on rare cases of bacteremia in humans caused by Pediococcus species (9, 10). hެT{0�W>�g�#V���Ȯ]!$�l �z��� Most of the previous cases used a series of biochemical tests to provide a species identification. 0000168491 00000 n It has been showed that CAT can extend lifespans by 20% in transgenic mice by increasing the enzyme levels through overexpression in various organelles [79, 80]. eCollection 2018 Apr. 0000076268 00000 n ΔCt represents the value after normalizing each target gene with the Ct value of the housekeeping gene. Sims W: The isolation of pediococci from human saliva. . 0000070933 00000 n 0000074604 00000 n 0000074524 00000 n Infect. Daudelin JF, Lessard M, Beaudoin F, Nadeau E, Bissonnette N, Boutin Y, Brousseau JP, Lauzon K, Fairbrother JM. 0000096254 00000 n Each of these patients had underlying predisposing conditions (two with cancer undergoing chemotherapy treatment [adenocarcinoma and acute myelogenous leukemia], one case of cutaneous Recklinghausen's disease, and one case of acute appendicitis with abdominal complications). These results suggested that the bacteriostasis of K88 was related to the organic acid and hydrogen peroxide produced by P25. Approximately 400 mg SiC particles (1 mm, BioSpec Products, Bartlesville, OK) were added to tubes and vortexed at maximum speed for 15 minutes to disrupt the worms. 0000073806 00000 n 0000100881 00000 n  |  0000074683 00000 n 0000079441 00000 n In addition, the longevity-regulating pathway-worm (LRP-W) ( value of 0.032241028) and longevity-regulating pathway-multiple species (LRP-MS) ( value of 0.035969185) associated with the lifespan of C. elegans were enriched. The mitogen-activated protein kinase (MAPK) pathway ( value of 0.099829268) associated with immune defense was also found. 0000071451 00000 n Fifteen valid Pediococcus species are recognized, most of them associated with plants and foods, although they are frequent colonizers of humans (J. P. Euzeby: List of Prokaryotic Names with Standing in Nomenclature, (11). Figures S1 and S2: the saturation and read homogeneity distribution analysis of the sequencing. P. acidilactici has a wide range of potential benefits which are still being studied. Brousseau JP, Talbot G, Beaudoin F, Lauzon K, Roy D, Lessard M. J Anim Sci. Get the latest research from NIH: *�5�8Ў)���|�[Sd�0A8� The supernatant was removed, and 0.9% NS was added to resuspend bacterial cells. 0000075710 00000 n Learn more about Institutional subscriptions. Pediococcus acidilactici and Pediococcus pentosaceus are the most common species associated with opportunistic infections affecting compromised hosts.

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